Dresden 2009 – wissenschaftliches Programm
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SYSO: Symposium Self-organizing Surfaces and Interfaces
SYSO 2: Self-Organizing Surfaces and Interfaces II
SYSO 2.2: Hauptvortrag
Mittwoch, 25. März 2009, 16:15–16:45, BAR SCHÖ
Active Organisation of Cell Surface Molecules by Cortical Actin — Kripa Gowrishankar1, Debanjan Goswami2, Subhasri Ghosh2, Abhishek Chaudhuri1, Bhaswati Bhattacharya3, Satyajit Mayor2, and •Madan Rao1,2 — 1Theoretical Physics, Raman Research Institute, Bangalore, India — 2National Centre for Biological Sciences, Bangalore, India — 3Theoretical Science Unit, Jawaharlal Nehru Centre for Advanced Scientific Research, Bangalore, India
The local organization of cell surface molecules is thought to be determined by thermodynamic forces. In particular, interactions with specific lipids are expected to result in protein complexes enriched in lipid-phases called `rafts'. I will briefly discuss our experimental work on the spatial distribution and steady state dynamics of a putative raft component, the GPI-anchored proteins, using high spatial and temporal resolution FRET microscopy. I will provide evidence that the spatial distribution and dynamics of reorganization of GPI-anchored proteins is regulated by the underlying cortical actin activity. I will next discuss a coarse-grained theoretical approach involving the dynamical interplay between membrane composition and cortical actin activity, based on the framework of active hydrodynamics. Apart from successfully capturing all features of our experimental results, we make testable predictions regarding the nature of fluctuations of cell surface GPI-anchored proteins. Finally, I will discuss consequences of such an active organisation on chemical reaction kinetics and spatial patterning in cell surface signaling.