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Q: Fachverband Quantenoptik und Photonik
Q 62: Photonik II
Q 62.9: Talk
Friday, March 6, 2009, 16:00–16:15, Audi-A
Chemical Nanonscopy of Artificial Membranes — •Fouad Ballout1, Henning Krassen2, Ilona Kopf1, Kenichi Ataka2, Erik Bründermann1, Joachim Heberle2, and Martina Havenith1 — 1Ruhr-Universität Bochum, Fakultät für Chemie & Biochemie, Lehrstuhl für Physikalische Chemie II — 2Universität Bielefeld, Fakultät für Chemie, Arbeitsgruppe Biophysikalische Chemie
Scanning near-field infrared microscopy (SNIM) provides a chemical and structural mapping of surface composition on the nanometer scale by combining the advantages of IR spectroscopy with the high spatial resolution of an atomic force microscope (AFM). This non-invasive, label-free imaging technique is of great interest in biology since it allows one to study biological materials by utilizing their chemical fingerprint without the need for dyes.
We have performed near-field measurements of an oriented membrane protein (Cytochrome c Oxidase) re-integrated in artificial lipid bilayers.
For the measurement, we used a LN2 cooled CO-Laser as an infrared radiation source covering the frequency range (1600-1800 1/cm), which includes the amide I band of the protein (1658 1/cm) and the C=O stretching mode of the lipids (1740 1/cm). Using a homebuilt scanning near-field microscope we were able to record simultanously nanoscale topography and near-field images as a function of laser frequency. An advanced image processing of the topography and the near-field image provided the evaluation of a frequency dependent contrast showing spectroscopic signatures.