Regensburg 2010 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 19: Membranes and Vesicles
BP 19.1: Vortrag
Mittwoch, 24. März 2010, 10:00–10:15, H43
Dynamics of endosomal population and cargo trafficking — •Jonathan Edward Dawson1, Lionel Foret3, Roberto Villasen2, Claudio Collinet2, Yannis Kalaidzidis2, Marino Zerial2, and Frank Jülicher1 — 1Max Planck Institute for the Physics of Complex Systems, Dresden — 2Max Planck Institute for Molecular Cell Biology and Genetics, Dresden — 3Ecole Normale Supérieure-LPS, Paris
Endosomes are vesicular structures that transport cargo molecules that are internalized into the cell by endocytosis. Endosomes exchange material by fusion and fission and form a cellular compartment in which cargo is sorted. We present a theoretical description of a population of endosomes. In the description we capture the cargo trafficking on a population level and take cargo influx, outflux, fusion and fission into account. Experimentally the cargo distribution in the endosomal population can be determined by fluorescence microscopy. Our theory predicts scaling properties of the network which are observed experimentally. The theory also describes characteristic features of steady state distribution of cargo molecules in Rab5 positive endosomes. We compare our theory to experimental data and determine the kinetic parameters of the early endosomal network in HeLa cells.