Regensburg 2010 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 34: Posters: New Technologies
BP 34.7: Poster
Donnerstag, 25. März 2010, 17:15–20:00, Poster B2
New Carbohydrate-based Protein Sensor Realized with Cantilever Arrays — •Kathrin Gruber1, Tim Horlacher2, Peter H. Seeberger2, and Bianca A. Hermann1 — 1CeNS and Walther-Meissner-Institute, Walther-Meissner-Str. 8, 85748 Garching, Germany — 2Max Planck Institute of Colloids and Interfaces, Department of Biomolecular Systems, Arnimallee 22, 14195 Berlin, Germany
Cantilever based detection opens new means for the label-free analysis of biomolecular interactions in real-time and up to eight channels. In the static operation mode, the biomolecular interaction is transduced into a deflection of a micrometer thin silicon beam that can be read-out with nanometer precision via optical beam deflection. Owing to recent advances in carbohydrate sequencing and synthesis, glycomics is catching up fast to the more established fields of genomics and proteomics. Measuring carbohydrate interactions is key to understand carbohydrate function requiring the development of reliable, sensitive and selective sensor surface chemistries. Cantilever array have been successfully used in gene fishing, single base pair recognition, and antigen-antibody assays. We design a purely carbohydrate based sensing layer to single out the central glycoconjugate recognition. Using a reference sensor to account for non-specific binding, we detect carbohydrate-protein interactions down to nanomolar concentrations. We verify the selective binding of proteins to carbohydrate functionalized cantilevers by a competitive inhibition assay. Our results pave the way for carbohydrate based cantilever sensors as a robust, scalable and label-free method to study medically relevant carbohydrate-protein interactions.