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BP: Fachverband Biologische Physik
BP 10: Posters: Tissue Dynamics \& Developmental Processes
BP 10.2: Poster
Monday, March 14, 2011, 17:15–20:00, P3
Novel Magnetic Tweezer with first Applications to Cell and Tissue Stimulation and Rheology — Claus Fütterer1 and •Rui Caldeira1,2 — 1University of Leipzig, Faculty of Physics and Earth Science Institute for Experimental Physics I, Soft Matter Physics Division, Developmental Biophysics, Leipzig, Germany — 2Universidade de Lisboa, Faculdade de Ciencias, Departamento de Fisica, Lissabon, Portugal
Studying biological samples with laser tweezers releases cosiderable heat perturbing eventually the sample. AFM requires a cantilever to approach the tip to the object in question. Magnetic fields in contrast do not disturb biological samples at all and it is possible to apply forces directly between superparamagnetic micro and nanoparticles applied to the sample in question without the need of immobilization. Those nano and microparticles have been extensively used to measure the visco-elastic properties on the cell membrane plus actin cortex. By switching perpendicular fields we found a new way to assemble those particles to a rich variety of macro-objects and to disassemble them again. 1. We discuss the objects which we found and explain the mechanism of stability. 2. We further discuss applications to study rheology of Hydra Vulgaris tissues in order to find out about the relation of visco-elastic properties and influence of mechanical stimulation onto the symmetry breaking transition during its development. This approach is well suited for high throughput assays in other applications.