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BP: Fachverband Biologische Physik
BP 10: Posters: Tissue Dynamics \& Developmental Processes
BP 10.7: Poster
Monday, March 14, 2011, 17:15–20:00, P3
Studying dynamical changes in lung parenchyma by using optical coherence tomography combined with confocal fluorescence microscopy — •Maria Gaertner1, Peter Cimalla1, Lilla Knels2, Sven Meissner1, Wolfgang M. Kuebler3, and Edmund Koch1 — 1TU Dresden, Faculty of Medicine Carl Gustav Carus, Clinical Sensoring and Monitoring, Dresden, Germany — 2TU Dresden, Faculty of Medicine Carl Gustav Carus, Department of Anatomy, Dresden, Germany — 3Institute for Physiology, Charité Berlin, Germany and and Department of Surgery, University of Toronto, Ontario
Realistic lung dynamical investigations on the alveolar microscale are hardly obtainable with conventional techniques such as light microscopy of tissue sections, micro computer tomography or magnetic resonance imaging due to preparation artifacts and damages of the sample or insufficient spatial and temporal resolution, respectively. Optical coherence tomography (OCT) as well as intravital microscopy provide noninvasive, high-resolution (µm), real-time (in 2D) imaging, capable of application to in vivo situations. Furthermore, OCT even extends the morphological information to three dimensions by successive recording of real-time two-dimensional cross-sections within a few seconds. As a new approach, the combination of OCT and confocal fluorescence microscopy shall not only provide 3D data of lung tissue but also localization of elastic fibers embedded in the biological structure through visualization of specifically binding fluorophores. Dynamic studies in an ex vivo mouse model allow for an estimation of overall elasticity as well as investigation of fiber rearrangements.