Dresden 2011 – scientific programme
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BP: Fachverband Biologische Physik
BP 10: Posters: Tissue Dynamics \& Developmental Processes
BP 10.8: Poster
Monday, March 14, 2011, 17:15–20:00, P3
Finite size corrections to scaling behavior in sorted cell aggregates — •Abigail Klopper1,2, Gabby Krens3, Stephan Grill1,2, and Carl-Philipp Heisenberg3 — 1Max Planck Institute for the Physics of Complex Systems, Nöthnitzer Straße 38, D-01187 Dresden, Germany — 2Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstraße 108, D-01307 Dresden, Germany — 3Institute of Science and Technology Austria, Am Campus 1, A-3400 Klosterneuburg, Austria
Cell sorting is a widespread phenomenon pivotal to the early development of multicellular organisms. In vitro cell sorting studies have been instrumental in revealing the cellular properties driving this process. However, these studies have as yet been limited to two-dimensional analysis of three-dimensional cell sorting events. Here we describe a method to record the sorting of primary zebrafish ectoderm and mesoderm germ layer progenitor cells in three dimensions over time, and quantitatively analyze their sorting behavior using an order parameter related to heterotypic interface length. We investigate the cell population size dependence of sorted aggregates and find that the germ layer progenitor cells engulfed in the final configuration display a relationship between total interfacial length and system size according to a simple geometrical argument, subject to a finite size effect.