Parts | Days | Selection | Search | Updates | Downloads | Help
BP: Fachverband Biologische Physik
BP 13: Posters: Biological Membranes
BP 13.2: Poster
Monday, March 14, 2011, 17:15–20:00, P3
Physical description of endosome dynamics — •Jonathan Edward Dawson1, Lionel Foret2, Claudio Collinet3, Roberto Villasen3, Yannis Kalaidzidis3, Lutz Brusch4, Andreas Deutsch4, Marino Zerial3, and Frank Jülicher1 — 1Max Planck Institute for the Physics of Complex Systems, Dresden, Germany — 2Ecole Normale Superieure, LPS, Paris, France — 3Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany — 4ZIH-TUD, Dresden , Germany
We present a theoretical study describing the collective dynamics of a endosomal population in a cell. Endosomes are vesicular structures that sort and transport cargo molecules that are internalized into the cell by endocytosis. Dynamics of endosomal trafficking and sorting involves a large number of individual endosomes which exchange material by fusion and fission thereby establish a dynamic network. Using fluorescence microscopy and automated image analysis we quantify cargo distributions in a specific endosomal network and present a general theory that provide a quantitative description of cargo trafficking in the network. The steady state distribution of total fluorescence intensity of cargo molecules in endosomes display a power law. Our theory can quantitatively reproduce the shape of steady distribution and their time dependence. We determine the kinetic parameters of early endosomal network in HeLa cells. Our theory predicts various scaling properties which have been observed in the experimental data.