Dresden 2011 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 20: Neurobiophysics
BP 20.2: Vortrag
Mittwoch, 16. März 2011, 10:45–11:00, ZEU 250
Optically Clamping Neurons in vitro — •Kai Bröking1,3,5, Ahmed ElHady1,2,4,5, Ragnar Fleischmann1, Theo Geisel1,3,4,5, Walter Stühmer2,4,5, and Fred Wolf1,3,4,5 — 1Max-Planck-Inst. für Dynamik und Selbstorganisation, Göttingen, Germany — 2Max-Planck-Inst. für experimentelle Medizin, Göttingen — 3Fakultät für Physik, Georg-August-Universität Göttingen — 4Bernstein Center for Computational Neuroscience, Göttingen — 5Bernstein Focus for Neurotechnology, Göttingen
Transfecting neurons to express the light-gated ion channel Channelrhodopsin2 (ChR2) makes it possible to influence their activity noninvasively, by means of photostimulation [1]. We have implemented a feedback control system using optical stimulation at λ ≈ 480 nm which can be used to regulate the firing rate of neural networks cultured on multielectrode arrays. Our system allows closed loop feedback on timescales comparable to those of synaptic response (1−5 ms). We present an experimental setup for adjusting the average firing rate of neurons by means of feedback controlled photostimulation, thus devising a way of optically clamping an ensemble of cells. [1] Boyden, E., et al., Nat Neurosci 8, 1263-1268, doi:10.1038/nn1525