DPG Phi
Verhandlungen
Verhandlungen
DPG

Dresden 2011 – wissenschaftliches Programm

Bereiche | Tage | Auswahl | Suche | Aktualisierungen | Downloads | Hilfe

BP: Fachverband Biologische Physik

BP 21: Biopolymers and Biomaterials II (with CPP)

BP 21.8: Vortrag

Mittwoch, 16. März 2011, 12:30–12:45, ZEU 260

Two-component Polymer Scaffolds for Controlled Three-dimensional Cell Culture — •Benjamin Richter1,2, Franziska Klein1, Thomas Striebel1, Clemens Franz1, Georg von Freymann3, Martin Wegener2, and Martin Bastmeyer11Zoologisches Institut, Karlsruher Institut für Technologie, 76131 Karlsruhe — 2Angewandte Physik, Karlsruher Institut für Technologie, 76131 Karlsruhe — 3AG Optische Technologien und Photonik, Technische Universität Kaiserslautern, 67663 Kaiserslautern

Fibrous collagen or matrigel matrices are commonly used to study three-dimensional (3D) cell behaviour, but these matrices have a random pore size and are structurally and chemically ill defined. We and others have recently shown that direct laser writing (DLW) is a versatile technique to fabricate tailored 3D cell-culture scaffolds in the micrometer to nanometer range. By using an adequate photoresist, elastic 3D scaffolds for cell-force measurements have also been realized. These DLW scaffolds have been homogeneously coated with ECM molecules. Ideally, they should rather have an adjustable distribution of cell-substrate contact sites to manipulate cell adhesion and cell shape in all three dimensions. By sequential DLW of two different photoresists, composite-polymer scaffolds with distinct protein-binding properties are fabricated and selectively bio-functionalised thereafter. Cells cultured in these scaffolds selectively form cell-adhesion sites with the functionalised parts, allowing for controlling cell adhesion and cell shape in 3D - forming the basis for future designer tissue-culture scaffolds.

100% | Mobil-Ansicht | English Version | Kontakt/Impressum/Datenschutz
DPG-Physik > DPG-Verhandlungen > 2011 > Dresden