Dresden 2011 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 34: New Technologies
BP 34.2: Vortrag
Freitag, 18. März 2011, 10:45–11:00, ZEU 260
Nanoscale Localisation of Adhesion Receptors and Binding Domains of Matrix Proteins Using Electric Field Microscopy — •Christina Müller1, Dimitar Stamov1, Carsten Werner1,2, and Tilo Pompe1 — 1Leibniz Institute of Polymer Research Dresden, 01069 Dresden, Germany — 2Center for Regenerative Therapies Dresden, 01307 Dresden, Germany
Biomaterial interfaces constitute the intersection of living tissues and artificial scaffolds. In order to reveal exogenous cues in guiding cell behaviour a precise localisation of adhesion receptors and their ligands at the extracellular matrix (ECM) at biomaterial interfaces is needed. Although several techniques allow for the determination of spatial protein distributions, most of them require specialised conditions (electron microscopy) or lack high resolution (optical microscopy). We applied scanning force microscopy to provide at the same time nanoscale resolution and easy access to biological samples at material interfaces. Using electric force microscopy and immunogold labelling we revealed the localisation of cell adhesion receptors, i.e. integrins, at ECMs reorganised by adherent cells. The modulated anchorage of the adhesion ligands by the polymer support lead to different reorganisation patterns of fibronectin fibrils at the adhesion sites together with differences in the distribution and density of integrins. By probing specific domains at fibronectin fibrils, we additionally found them in a stretched state caused by the involved receptor forces. The versatility and straightforward implementation suggests the used method to further characterise different sets of ECM structures and corresponding receptors.