Dresden 2011 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 34: New Technologies
BP 34.6: Vortrag
Freitag, 18. März 2011, 12:00–12:15, ZEU 260
Combined 3D structural and molecular imaging using optical coherence tomography and laser scanning microscopy — •Maria Gaertner1, Peter Cimalla1, Lilla Knels2, Sven Meissner1, Wolfgang M. Kuebler3, and Edmund Koch1 — 1TU Dresden, Faculty of Medicine Carl Gustav Carus, Clinical Sensoring and Monitoring, Dresden, Germany — 2TU Dresden, Faculty of Medicine Carl Gustav Carus, Department of Anatomy, Dresden, Germany — 3Institute for Physiology, Charité Berlin, Germany and and Department of Surgery, University of Toronto, Ontario
Since the early 1990s, optical coherence tomography (OCT) has an emergent impact on biomedical and biophysical research. As a noninvasive optical technique, it provides three-dimensional, contactless, high-resolution (µm) imaging of tissue substances with penetration depths of up to several millimeter. Exploiting its abilities, in vivo histological studies become feasible without extraction of biological tissue. The sample’s morphology can easily be obtained within a few milliseconds. Apart from all its benefits, the lack of molecular specific interactions limits this method to a mere coarse investigation of tissue architecture. Utilizing laser scanning microscopy, the detailed molecular structure of biological samples can be obtained via specifically binding dyes to the substance of interest. In this study, we present a combined setup for simultaneous OCT and confocal fluorescence microscopy, allowing fast three-dimensional imaging of lung morphology and detection of elastic fiber distributions arising from the biomolecule elastin within lung tissue.