Dresden 2011 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 5: Tissue Dynamics \& Developmental Processes
BP 5.3: Vortrag
Montag, 14. März 2011, 14:45–15:00, ZEU 250
Cell flow reorients planar cell polarity in the developing wing epithelium of the fly — •Douglas B. Staple1, Reza Farhadifar1, Benoît Aigouy2, Andreas Sagner2, Jens-Christian Röper2, Suzanne Eaton2, and Frank Jülicher1 — 1Max Planck Institute for the Physics of Complex Systems, Dresden, Germany — 2Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany
Epithelia are two-dimensional sheets of cells. Cell polarity in epithelia typically forms large scale aligned patterns in the plane of the tissue. In the Drosophila wing, an important model system for the study of epithelial organization, this planar polarity is reflected in the pattern of wing-hairs and in the distribution of planar cell polarity (PCP) proteins at earlier stages during development. Here we investigate the mechanisms underlying the dynamic reorganization of planar cell polarity in the Drosophila wing using a combination of theory and experiment. Experimentally, we perform time-lapsed imaging during pupal development in order to extract both the time-dependent distribution of PCP proteins, and also the spatially and temporally inhomogeneous cell flow field in the tissue. The pattern of PCP proteins is found to reorient during development. We decompose the velocity field into patterns of local shear, compression, and rotation rates. Given the time-dependent shear and rotation rates and an experimentally measured initial condition, the time-evolution of the polarity pattern is computed using a phenomenological hydrodynamic theory, and is found to be consistent with the experimentally observed time-evolution.