Dresden 2011 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 9: Posters: DNA \& DNA Enzymes
BP 9.2: Poster
Montag, 14. März 2011, 17:15–20:00, P3
Synthesis of covalently linked DNA structures — •Anja Henning1,2, Ofer I. Wilner2, Bella Shlyahovsky2, Michael Mertig1, and Itamar Willner2 — 1Professur für Physikalische Chemie, Mess- und Sensortechnik, TU Dresden, 01062 Dresden, Germany — 2Institute of Chemistry and The Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, Jerusalem 91904, Israel
The ability of DNA as a material for bottom-up approaches has been shown already in an enormous number of experiments. Apart from some exceptions, the principle behind such DNA self-assemblies is the hybridization of complementary sequences through Watson-Crick base pairing which is unstable upon heating. We developed a new method to synthesize thermostable 2D and 3D DNA nanostructures by connecting single-stranded DNA (ssDNA) parts via covalent bonds. In order to demonstrate this approach, we used a ssDNA circle that contained four different internal modifications on its poles. These circles were cross-linked via the formation of covalent bonds with a ssDNA molecule that includes a modification on its 3` and 5` ends. We performed experiments using a circle with four amine functionalities and alternatively a circle, that contained thiol and amine functionalities at its opposite poles to yield DNA nanotubes. The single-stranded approach makes those structures suitable to guide patterning of nanoparticles, proteins and transition metals. Furthermore, the stability upon heating gives an outstanding erase/rewrite functionality, providing the possibility of a controlled release of the attached nanomaterials.