Dresden 2011 – wissenschaftliches Programm
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CPP: Fachverband Chemische Physik und Polymerphysik
CPP 2: Biopolymers and Biomaterials I (jointly with BP)
CPP 2.6: Vortrag
Montag, 14. März 2011, 12:00–12:15, ZEU 222
The swelling/stability effect of hyaluron on a lipid multilayer system — •Martin Kreuzer1, Markus Strobl2, Matthias Reinhardt2, Reiner Dahint1, and Roland Steitz2 — 1Universität Heidelberg, Physikalisch Chemisches Institut, 69120 Heidelberg, Germany — 2Helmholtz-Zentrum Berlin GmbH, 14109 Berlin, Germany
Hyaluron (HA) is a high molecular weight polysaccharide. HA is involved in a wide range of processes in the human body, such as wound healing, severe stress, tumor progression and invasion. It was possible to show, that HA also stabilizes lipid multilayer systems at physiological conditions: Neutron reflectometry measurements, carried out at V6 and BioRef neutron reflectometer at the Helmholtz-Zentrum Berlin, in excess D2O verified, that a oligolamellar DMPC lipid bilayer coating remains stable on a silicon substrate at 21°C in its ordered state (Lβ) with a d-spacing of 66Å, but detaches almost completely at 38°C in its chain-disordered Lα state from the solid support - the origin of the loss of the oligolamellar DMPC bilayer stack at 38°C is unclear. By contrast oligolamellar lipid bilayers remain stable on a substrate at 38°C when incubated with a solution of HA in D2O: In an independent experiment, an oligolamellar lipid bilayers stack was measured against a solution of 3mg/mL HA in D2O. The sample was investigated shortly after incubating at 21°C and after raising sample temperature to 38°C. The oligolamellar lipid layer remained stable on the substrate, but an immense swelling occurred until a d-spacing of 209Å is reached. We will discuss a possible mechanism of the transformation of the oligolamellar lipid system with incubation time.