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MO: Fachverband Molekülphysik
MO 24: Poster: Femtosecond spectroscopy
MO 24.16: Poster
Thursday, March 17, 2011, 16:00–18:00, P2
The Phycocyanobilin-Derivative AIE as Model System for the Pfr Photoreaction in Phytochromes — •Patrick Singer1, Sonja Fey2, Gudrun Hermann2, and Rolf Diller1 — 1Fachbereich Physik, TU Kaiserslautern, D-67663 Kaiserslautern — 2Institut für Biochemie und Biophysik, Friedrich-Schiller-Universität Jena, D-07743 Jena
An insufficiently treated issue in the phototransformation of phytochromes concerns both the bathochromic shift of the ground state absorption as well as the much faster primary photoreaction of the far-red absorbing state Pfr as compared to the red absorbing state Pr. The lactimderivative of phycocyanobilin, phycocyanobilindimethylester-ring-A-monomethylimino-ester (AIE), has been suggested to mimic these static and dynamic properties of the phytochrome Pfr state, i.e. the phytochrome bilin chromophore interacting with specific sites in the protein binding pocket of Pfr [1]. We have used femtosecond transient absorption spectroscopy to investigate the ultrafast light-induced primary processes of AIE in methanol. The results are discussed in the context of previously observed dynamics and absorption shifts of the Pfr state in phytochromes [2,3].
[1] R. Micura and K. Grubmayr Angew. Chem. 107, 1896-2522 (1995)
[2] C. Schumann, R. Groß, M. M. N. Wolf, R. Diller, N. Michael, and T. Lamparter. Biophys. J. 94, 3189-3197 (2008)
[3] Bischoff, M., G. Hermann, S. Rentsch, and D. Strehlow Biochemistry 40, 181-186 (2000)