Dresden 2011 – wissenschaftliches Programm
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O: Fachverband Oberflächenphysik
O 17: Metal substrates: Adsorption of organic / bio molecules III
O 17.4: Vortrag
Montag, 14. März 2011, 18:00–18:15, TRE Phy
Scanning Tunneling Microscopy of Proteins in Ultrahigh Vacuum — •Stephan Rauschenbach1, Nicha Thontasen1, Deng Zhitao1, Rinke Gordon1, and Kern Klaus1,2 — 1Max-Planck-Institute for Solid State Research, Stuttgart, Germany — 2Institut de Physique de la Matiere Condensee, Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland
Proteins are biosynthesized, self-assembled, functional polymers of unprecedented complexity and specify in their function. Since the shape which determines their behavior depends strongly on the environment they typically are investigated under physiological conditions. The functionalization of well defined surfaces with such molecules would be an important step towards using high performance vacuum based techniques, like scanning probe microscopes. Here we show the controlled deposition and in situ characterization of intact proteins on clean metal surfaces in ultrahigh vacuum by electrospray ion beam deposition (ES-IBD). We demonstrate that depending on the charge state, the protein can be deposited in a folded or unfolded state. For unfolded proteins, scanning tunneling microscopy reaches single amino acid level resolution.