Dresden 2011 – wissenschaftliches Programm
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O: Fachverband Oberflächenphysik
O 36: Poster Session II (Metals; Nanostructures at surfaces; Surface or interface magnetism; Spin-Orbit Interaction at Surfaces; Electron and spin dynamics; Surface dynamics; Methods; Theory and computation of electronic structure)
O 36.5: Poster
Dienstag, 15. März 2011, 18:30–22:00, P4
Specific protein patterning in protein-repelling monomolecular matrix by UV promoted exchange reaction — •Jeyachandran Yekkoni1, Andreas Terfort2, and Michael Zharnikov1 — 1Angewandte Physikalische Chemie, Universität Heidelberg, 69120 Heidelberg, Germany. — 2Institut für Anorganische und Analytische Chemie, Universität Frankfurt, Max-von-Laue-Straße 7, 60438 Frankfurt, Germany.
Oligo(ethylene glycol) (OEG) self assembled monolayers, well known for their protein resistance behaviour, could be used to produce protein patterns upon introduction of specific receptor groups by exchange reaction enabled by controlled degradation. We investigated the degradation of OEG monolayers using UV irradiation at 254, 312, and 366 nm to achieve well-controlled exchange reaction and protein patterning. OEG molecules with different lengths of the alkyl and OEG stems as well as with different tail groups (OCH3 or OH) were used to prepare monolayers on Au surface and biotinylated alkanethiol was used as a receptor for avidin or streptavidin. Experiments at zero dose showed that the OH terminated OEG monolayers with a sufficiently long alkyl linker and the EG chain (minimum 5 units) are stable towards non-specific receptor exchange reaction for at least 5 min. incubation. At 254 nm irradiation the degradation kinetics of the OEG chains was very fast that produced complete degradation (direct patterning) at dose below 8 J/cm2. However, 312 and 366 nm irradiation provided the possibility to control the degradation (defects formation) at low doses followed by direct patterning at doses greater than 103 J/cm2.