Berlin 2012 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 17: Posters: Physics of Cells

BP 17.41: Poster

Mittwoch, 28. März 2012, 17:30–19:30, Poster A

Synchrotron-based functional imaging of prostate tissue samples — •Andrea Matschulat¹, Georgi Graschew², Anton Serdyukov¹, Arne Hoehl¹, Ralph Müller¹, and Gerhard Ulm¹ — ¹Physikalisch-Technische Bundesanstalt (PTB), Abbestr. 2-12, 10587 Berlin, Germany — ²ECRC Campus Buch, Max-Delbrück-Centre of Molecular Medicine, Lindenberger Weg 80, 13125 Berlin, Germany

In our contribution we focus on the sensitive biochemical and functional imaging of human tissue samples with the help of synchrotron-based FTIR-microspectroscopy. FTIR-microspectroscopy is an optical non-invasive technique and has proven to be a fast diagnostic readout tool for multiplexed analysis of biomolecules, mainly in the biomedical field. Measurements were performed at the Metrology Light Source (MLS) of the PTB, an unique low-energy electron storage ring that provides broad-band synchrotron radiation. In our systematic approach we studied the MIR signatures of human benign and malign prostate tissue samples in the spectral range from 900 cm^-1 to 3900 cm^-1 under implementation of brilliant synchrotron radiation and globar source in the reflection mode. For a reliable clinical diagnosis with respect to distinct localization of benign and malign tissue areas, a sensitive detection of tissue samples with/without biomarkers is very important. Additionally, spectral quality, i.e., high S/N-ratios in spectral datasets and their correction by Mie scattering algorithms are needed. We will discuss the results of Mie-corrected MIR signatures of prostate tissues and evaluate their classification by multivariate statistics.