Berlin 2012 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 6: Physics of Cells II
BP 6.6: Vortrag
Montag, 26. März 2012, 16:30–16:45, H 1028
Tailored Micro-Stencils for studying the impact of size and shape of migrating cell ensembles on their collective behavior — •Sebastian Rausch1,2, Christian H. J. Böhm1,2, and Joachim P. Spatz1,2 — 1Max Planck Institute for Intelligent Systems, Department of New Materials and Biosystems, Stuttgart — 2Heidelberg University, Institute for Physical Chemistry, Heidelberg
Many fundamental biological processes, including morphogenesis, tissue repair and tumor invasion, require the collective motions of cells within groups, that are connected by cell-cell junctions.
The objective of our work is to elucidate and characterize the collective behavior of cell ensembles addressing the following key questions: To which extent are cell systems capable of developing multi-cellular collective patterns? How are collective properties established and controlled? We are thereby particularly interested in the formation of so called leader-cells and the specific impact of size and shape of a cell ensemble on its collective behavior and emergent properties.
Here, we introduce a tailored and adapted micro-stencil-technique based on soft-lithography, which allows us to create cell ensembles of arbitrary and well-defined shape and size. Our approach enables us to quantify the impact of these crucial boundary conditions. This technique enables us to improve the understanding of collective cell migration mode. Thorough knowledge of this fundamental migration behavior is a necessary prerequisite for a comprehensive understanding of morphogenesis or tumor metastasis via collective migration.