Berlin 2012 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 7: Posters: Proteins

BP 7.27: Poster

Montag, 26. März 2012, 17:30–19:30, Poster A

The Impact of Salt and Pressure on the Interaction Potential of Proteins in Solution — •Johannes Möller¹, Martin A Schroer¹, Mirko Erlkamp², Sebastian Grobelny², Michael Paulus¹, Andre Steffen¹, Sebastian Tiemeyer¹, Florian J Wirkert¹, Metin Tolan¹, and Roland Winter² — ¹Fakultät Physik/DELTA, TU Dortmund, Otto-Hahn-Str. 4, 44227 Dortmund — ²Physikalische Chemie, TU Dortmund, Otto-Hahn-Str. 6, 44227 Dortmund

The fabrication of crystals from protein solution has become the defining task in obtaining high resolution protein structures by X-ray diffraction. Here, the use of salt in the solution is commonly known to increase attractive interactions and therefore *salting out* the proteins. Nevertheless, the fabrication of high quality crystals is still a challenging task, due to the many different influences on the interactions in the protein solution, e.g. temperature, ionic strength, protein concentration, and pressure. We present the results of Small Angle X-ray Scattering experiments, which reveal the combined influence of hydrostatic pressure and ionic strength on the interaction potential of dense protein solution. Here, a more precise knowledge about the interaction between proteins in solution can help to predetermine possible crystallization conditions, which is even more important when only a small amount of protein is available.