Berlin 2012 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 7: Posters: Proteins
BP 7.8: Poster
Montag, 26. März 2012, 17:30–19:30, Poster A
Chloroplast fluorescence excitation and emission spectroscopy in live plant cells — •Sebastien Peter1, Martina Zell5, Christian Blum3, Kirstin Elgass4, Veronica Maurino5, Alfred Meixner2, Vinod Subramaniam3, and Frank Schleifenbaum1 — 1Zentrum für Molekularbiologie der Pflanzen, Universität Tübingen, Deutschland — 2Institut für physikalische und theoretische Chemie, Universität Tübingen, Deutschland — 3Biophysical Engineering, University of Twente, Enschede, The Netherlands — 4Biochemistry Department, La Trobe University, Bundoora, Australia — 5Botanisches Institut, Universität zu Köln, Deutschland
Statistical analysis of chloroplast fluorescence spectra recorded at room temperature enables for drawing conclusions about the relative PSI/PSII ratio in wild type and carbon deficient plant cells. Leaf cells exhibit fluorescence from chloroplasts whose spectra show two bands assigned to the photosystems. Changes in a plant's environmental conditions are reflected by an altered efficiency of photosynthesis. Fluorescence excitation spectra of plants grown under different conditions show three peaks assigned to different carotenoids. The spectra not only differ in the intensity ratios between different pigments but also in the broadth of their distributions revealing a higher adaptation flexibility of wild-type plants. Therefore, fluorescence excitation and emission spectroscopy at room temperature enables for live read-out of the photosynthesis adaption to external conditions without generating artifacts from extensive sample preparation and low temperatures.