Berlin 2012 – wissenschaftliches Programm
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CPP: Fachverband Chemische Physik und Polymerphysik
CPP 7: Poster: Biopolymers and Biomaterials (jointly with BP)
CPP 7.4: Poster
Montag, 26. März 2012, 17:30–19:30, Poster A
Frequency resolved depolarized light scattering studies of tRNA conformation in solution — •Christoph Angermann1 and Thomas Hellweg2 — 1Universität Bayreuth, Physikalische Chemie 1 — 2Universität Bielefeld, Physikalische und Biophysikalische Chemie
A custom built depolarized dynamic light scattering apparatus equipped with a confocal Fabry-Perot interferometer (FPI) with a free spectral range of 150 MHz was developed. The FPI is used to analyze the scattered light in the frequency domain. This is an important characteristic because the rotational motion of the particles modifies the depolarized component of the scattered light. Due to further development of this setup, it is easily possible to accumulate the noisy component efficiently leading to an enhancement of the signal to noise ratio. Combined with an iterative deconvolution method this apparatus can be used to analyze the rotational diffusion of tRNA from brewer's yeast in solution in a time range of 1 to 80 ns.