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Berlin 2012 – wissenschaftliches Programm

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CPP: Fachverband Chemische Physik und Polymerphysik

CPP 9: Biopolymers and Biomaterials (joint session with BP)

CPP 9.8: Vortrag

Dienstag, 27. März 2012, 11:45–12:00, H 1058

Direct Laser Writing for Three-dimensional Biological Application — •Benjamin Richter1,2, Alexandra Greiner1, Clemens Franz1,2, Martin Wegener2,3,4, and Martin Bastmeyer1,21Zoologisches Institut, Karlsruher Institut für Technologie, 76131 Karlsruhe — 2DFG-Center for Functional Nanostructures (CFN), Karlsruher Institut für Technologie, 76131 Karlsruhe — 3Angewandte Physik, Karlsruher Institut für Technologie, 76131 Karlsruhe — 4Institut für Nanotechnologie, Karlsruher Institut für Technologie, 76021 Karlsruhe

Direct laser writing (DLW) is a versatile technique to fabricate tailored three-dimensional (3D) cell-culture scaffolds in the micrometer to nanometer range. By sequential DLW of two different photoresists, composite-polymer scaffolds with distinct protein-binding properties are fabricated and selectively bio-functionalized thereafter. Cells cultured in these scaffolds selectively form cell-adhesion sites with the functionalized parts, allowing for controlling cell adhesion and cell shape in 3D * forming the basis for future designer tissue-culture scaffolds. To go one step further photoactivation of 3D scaffolds might play an important role in the future. One application realized by using these two-component polymer scaffolds is measuring forces of cells in a three-dimensional environment. With our technique we can control the number, size, and geometry of adhesive cubes. The forces induced by a single cell onto the scaffolds are proportional to the bending of the beams.

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