Berlin 2012 – scientific programme
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MI: Fachverband Mikrosonden
MI 6: X-ray imaging, holography and tomography
MI 6.3: Talk
Wednesday, March 28, 2012, 13:00–13:15, TA 201
Investigation of biological specimens with a 30 nm resolution table top X-ray microscope based on a high repetition laser plasma source — •Christian Seim1, Christoph Redlich1, Jonas Baumann1, Gernot Blobel2, Herbert Legall2, Bernhard Hesse3, Holger Stiel2,4, and Birgit Kanngießer1,4 — 1TU Berlin, Germany — 2Max-Born-Institute, Germany — 3Chartité Berlin, Germany — 4Berlin Laboratory for innovative X-ray Technologies (BLiX), Germany
This talk describes a table top X-ray microscope based on a high repetition rate laser plasma source which enables investigations of biological specimens at 30 nm resolution. The laboratory soft X-ray microscope works at the nitrogen Lyα at 2.478 nm in the so called water window, to gain a high contrast when investigating biological samples with thickness up to 10 µm. A detailed description of the instrument is given and the feasibility of X-ray tomography of aqueous samples will be discussed. First images of reference biological samples, diatoms, with structures in the 30 nm region will be shown. It is planned to apply the instrument for investigations of the BRONJ disease. BRONJ is a rare disease, in which the patient’s jaw bone suffers osteonecrosis, induced by bisphosphonates (Salvatore L. Ruggiero et al., 2009). One theory is that the canaliculi system that connects and supplies the osteocytes (bone cells) becomes clogged. The canaliculi diameter is approximately 300 nm. The feasibility of capturing a 3D image of the canaliculi network using X-ray tomography will be discussed.