Berlin 2012 – wissenschaftliches Programm
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O: Fachverband Oberflächenphysik
O 13: Polymeric biomolecular films
O 13.3: Vortrag
Montag, 26. März 2012, 16:30–16:45, MA 005
X-ray reflectivity measurements of lipid membranes at the water/oil interface — •Annika Elsen1, Lars Joergensen2, Klaas Loger1, Benjamin Runge1, Oliver H. Seeck3, Beate Klösgen2, Olaf M. Magnussen1, and Bridget M. Murphy1 — 1Institute for Experimental and Applied Physics, CAU, Kiel, Germany — 2MEMPHYS, Department of Physics, Chemistry and Pharmacy, SDU, Odense, Denmark — 3PETRA III, DESY, Hamburg, Germany
For a better understanding of the function of biological membranes the arrangement of the lipids in the bilayer as well as the structural integration of the proteins, providing the functions of the membrane, has to be known. In a first attempt a biological membrane can be modelled with phosphocholine monolayers at the water/oil interface. Unfortunately the application of structure-sensitive techniques on membranes in such environment is limited. However, with X-ray diffraction techniques it is possible to obtain structural information on the membrane at the liquid/liquid interface on a sub-molecular scale. Here, we present X-ray reflectivity measurements of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) monolayers at the water/perfluorohexane interface, the latter with different bulk concentrations, carried out at the new liquid interface scattering apparatus (LISA) at the beamline P08 (PETRA III, DESY). Preliminary results of the vertical monolayer structure at the interface will be presented, giving information on the arrangement of the lipids.