Stuttgart 2012 – wissenschaftliches Programm
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MO: Fachverband Molekülphysik
MO 11: Biomolecules
MO 11.8: Vortrag
Dienstag, 13. März 2012, 15:45–16:00, V38.02
Multiplex-CARS microspectroscopy as a tool for fast characterisation of ligno-cellulosic biomass — •Christoph Pohling1, Christian Brackmann2, Tiago Buckup1, Annika Enejder2, and Marcus Motzkus1 — 1PCI, Uni Heidelberg, Germany — 2Dep. of Chemical and Biological Engineering, Chalmers University, Göteborg, Sweden
Wood biopolymers such as cellulose and lignin have strong potential to replace petroleum-based sources for synthesis of chemicals and materials. This development requires efficient characterization of these renewable raw products, especially in industrial processing where measurement speed is an important factor. Raman microspectroscopy detects sample components probing molecular vibrations. However, the method is time consuming and a pixel dwell time of 500 ms is often necessary in case of biological samples. In this work acquisition time has been reduced using the non-linear Raman-based technique multiplex coherent anti-Stokes Raman scattering (MCARS) microscopy. Wood samples have been investigated using a data acquisition time of 60 ms per pixel and MCARS provided excellent image contrast, background-free chemical imaging and quantitative results. Data analysis using the maximum entropy method (MEM) gave Raman-equivalent data on sample composition and polarization control of the excitation beams provided additional information on the molecular orientation of the wood fibers. In total, MCARS turned out to be a very suitable tool for material characterization, providing rapid imaging with quantitative measures of sample composition and structural information.