Hannover 2013 – wissenschaftliches Programm
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MO: Fachverband Molekülphysik
MO 27: Poster 3: Collisions, Electronic Spectroscopy, Energy Transfer, Quantum Chemistry, Molecular Dynamics, Photochemistry, Spectroscopy in He-Droplets
MO 27.28: Poster
Donnerstag, 21. März 2013, 16:00–18:30, Empore Lichthof
Single Molecule Fluorecence Excitation Spectroscopy on the B800 band of LH2 Complexes from Allochromatium Vinosum — •Alexander Löhner1, Richard J. Cogdell2, and Jürgen Köhler1 — 1Experimental Physics IV, and Bayreuth Institute for Macromolecular Research (BIMF), University of Bayreuth, Germany — 2Institute of Molecular, Cell & Systems Biology College of Medical Veterinary and Life Sciences, University of Glasgow, United Kingdom
The peripheral light-harvesting complex (LH2) from the photosynthetic purple bacterium Allochromatium vinosum features an unusual absorption spectrum in the 800 nm range, with respect to the more commonly studied LH2 complex from Rhodopseudomonas acidophila. While the latter species feature a single broad band in this spectral range, the absorption is split into two components for Allochromatium vinosum.
We have recorded fluorescence excitation spectra of the B800 band from 71 single LH2 complexes from the species Allochromatium vinosum at low temperature. The spectra were analysed with respect to the number of bands, the relative intensities of the bands and the spectral intra- and intercomplex heterogeneity. As a result, we can rule out that the splitting of the B800 band in an ensemble of LH2 complexes reflects the superposition of spectra from two different types of peripheral light-harevsting complexes.