DPG Phi
Verhandlungen
Verhandlungen
DPG

Regensburg 2013 – scientific programme

Parts | Days | Selection | Search | Updates | Downloads | Help

BP: Fachverband Biologische Physik

BP 13: Imaging

BP 13.3: Talk

Tuesday, March 12, 2013, 10:15–10:30, H44

STED Microscope with Spiral Phase Contrast — •Marcel Andreas Lauterbach1, Marc Guillon1, Asma Soltani2, and Valentina Emiliani11University Paris Descartes, Paris Sorbonne Cité, Neurophysiology and New Microscopies Laboratory, Paris, France — 2University Paris Descartes, Paris Sorbonne Cité, Laboratory of Membrane Dynamics and Neurological Diseases, Paris, France

We present a STED (STimulated Emission Depletion) microscope with phase contrast imaging capabilities. Phase contrast permits the visualization of weak phase objects without any labeling. It is implemented without extra optical elements in the STED light path, which would compromise STED imaging capabilities. Scanning phase contrast allows for registration with the fluorescence images and principally for simultaneous recording of phase contrast and STED images.

Phase contrast images of objects as thin as a few nanometers in optical path mismatch are obtained. The method provides a second imaging channel in a STED microscope for phase contrast imaging even in index-matched mounted cell cultures. This allows for correlation of morphological structures with high-resolution fluorescence images.

100% | Mobile Layout | Deutsche Version | Contact/Imprint/Privacy
DPG-Physik > DPG-Verhandlungen > 2013 > Regensburg