DPG Phi
Verhandlungen
Verhandlungen
DPG

Regensburg 2013 – wissenschaftliches Programm

Bereiche | Tage | Auswahl | Suche | Aktualisierungen | Downloads | Hilfe

BP: Fachverband Biologische Physik

BP 13: Imaging

BP 13.9: Vortrag

Dienstag, 12. März 2013, 11:45–12:00, H44

Quantifying Lipid and Protein Diffusion in Black Lipid Membranes — •Kerstin Weiß and Jörg Enderlein — Drittes Physikalisches Institut, Georg-August-Universität Göttingen, Göttingen, Germany

Lipid diffusion is crucial in many biological processes. However, accurately determining diffusion coefficients in membranes remains difficult. We used dual-focus fluorescence correlation spectroscopy (2f-FCS) to measure lipid diffusion in black lipid membranes (BLMs), i.e. lipid bilayers spanned over a pore. To generate these bilayers a commercially available setup is employed. We first tested the effect of different ionic strengths in neutral and negatively charged bilayers. Moreover, we investigated the influence of mono- vs. divalent ions. While monovalent ions have no effect on lipid diffusion in both systems, addition of divalent ions severely decreased the lipid*s diffusion coefficient in negatively charged BLMs. Furthermore, the investigated the validity of the Saffman-Delbrück model which is used to describe protein diffusion in lipid membranes.

100% | Mobil-Ansicht | English Version | Kontakt/Impressum/Datenschutz
DPG-Physik > DPG-Verhandlungen > 2013 > Regensburg