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Regensburg 2013 – scientific programme

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BP: Fachverband Biologische Physik

BP 24: Posters: Physics of Cells

BP 24.7: Poster

Wednesday, March 13, 2013, 17:30–19:30, Poster C

Elasticity measurements of fibroblastic cell nuclei by atomic force microscope for characterizing Lamin and TMEM43 mutations — •Tamara Münnich1, Volker Walhorn1, Helene Schellenberg1, Astrid Kassner2, Hendrik Milting2, and Dario Anselmetti11Experimental Biophysics, Bielefeld University, Germany — 2Erich und Hanna Klessmann-Institut, Herz- und Diabeteszentrum Bad Oeynhausen, Germany

Arrhythmogenic right ventricular cardiomyopathy (ARVC) is an inherited heart muscle disease associated with cardiac arrhythmia. It is a major cause of sudden cardiac death in the young and athletes. Mutations of the intermediate filament Lamin and the transmembrane protein 43 (TMEM43) are connected to ARVC. Both proteins are located at the nuclear envelope. As the cell nucleus has to resist strong mechanical stress caused by the contraction of the heart muscle, we suppose that the mutations affect functional mechanical properties of the nucleus. The elastic moduli of the cell nuclei were measured with the atomic force microscope and estimated by the Hertz-model. The measurements were performed with fibroblasts, which serve as a model system for cardiomyocytes. We analyzed a set of Lamin and TMEM43 mutated cells and compared them to a control group consisting of wild type fibroblasts and mutations not associated with ARVC. The TMEM43 mutant showed much higher and widespread elastic moduli, whereas the elasticity of the Lamin mutant is similar to the control group. In the future we will analyze modified fibroblasts expressing no Lamin and TMEM43 respectively.

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