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Regensburg 2013 – scientific programme

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BP: Fachverband Biologische Physik

BP 25: Posters: Cytoskeleton

BP 25.13: Poster

Wednesday, March 13, 2013, 17:30–19:30, Poster C

Passive Microrheology: A model-based approach to determine mechanical properties of assembled networks — •Tobias Paust1, Ines Martin1, Michael Beil2, and Othmar Marti11Institute for Experimental Physics, Ulm University — 2Department of Internal Medicine I, Ulm University

Macro- and microrheology is extensively used to characterize complex networks of biopolymers. A possible way to describe the mechanical properties of a viscoelastic medium is to measure the thermal motion of a particle embedded in the medium and compute the unilateral transformation.

In a model-based approach the mean squared displacement of the particle motion is divided into three terms describing the different properties of the network - elasticity, viscosity and the motion of the particle in a spatial confinement.

The focus of our work are the measurements of in vitro assembled keratin 8/18 networks to underline the functionality of the model-based approach. Furthermore, Brownian motion simulations of spatially confined beads with the influence of the meshes' elasticity and its drift are performed to allow a comparison to the measurements.

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