Regensburg 2013 – wissenschaftliches Programm
Bereiche | Tage | Auswahl | Suche | Aktualisierungen | Downloads | Hilfe
BP: Fachverband Biologische Physik
BP 8: Posters: Proteins
BP 8.30: Poster
Montag, 11. März 2013, 17:30–19:30, Poster B2
Pitfalls and artifacts in two-focus fluorescence fluctuation spectroscopy — •Andreas Veres and Matthias Weiss — Experimental Physics I, University of Bayreuth, Germany
Fluorescence fluctuation spectroscopy is a powerful technique to quantify intracellular transport on the almost single-molecule scale. Going beyond a single observation volume, temporally cross-correlating the fluorescence from two separated foci can be exploited to obtain large-scale transport coefficients, and to detect transport barriers or directed flow. Using mean-field theory and simulations in combination with experiments on a laser scanning microscope (LSM), we have investigated which artifacts and pitfalls may hamper this promising approach. We find that the unavoidable bleaching of fluorophores only has a minor influence on the results, whereas the typically poor statistics in commercial LSMs severely limits the applicability of the approach to in vitro systems.