Dresden 2014 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 1: Molecular Motors
BP 1.11: Vortrag
Montag, 31. März 2014, 12:30–12:45, HÜL 386
Linking single-motor dynamics to ciliary ultrastructure using single-molecule super-resolution microscopy in Caenorhabditis elegans. — •Felix Oswald1, Bram Prevo1, Pierre Mangeol1, Jonathan Scholey2, and Erwin Peterman1 — 1VU University Amsterdam, Amsterdam, Netherlands — 2University of California, Davis, Davis, United States of America
Cilia are finger-like protrusions that are present in most eukaryotic cells fulfilling crucial motility and sensory functions. Their shape and structural integrity is determined by microtubules and a variety of other structural components such as transition fibers and Y-shaped links. In Caenorhabditis elegans two kinesin-2-family motors, kinesin-II and OSM-3, are responsible for building and maintenance of the chemosensory cilia. It is known that both kinesins are active on the microtubule doublets and that OSM-3 alone maintains the microtubule singlets. It is not known, however, how other structural features influence their dynamics. In order to address this question we use single transgenes encoding for fluorescently labeled kinesins in combination with single-particle tracking PALM (sptPALM). By localizing single-motor proteins deep inside the living organism we are able to build super-resolution roadmaps that reveal ultrastructural details. This allows us to relate the rich single-motor dynamics to ciliary subdomains and their specific structural features. Our findings are the outset to understand the influence of ciliary ultrastructure on motor dynamics.