Dresden 2014 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 14: Posters: Protein Structure and dynamics
BP 14.10: Poster
Dienstag, 1. April 2014, 09:30–12:30, P1
Influence of surface and subsurface properties on the structure and activity of adsorbed bactericidal proteins — •Christian Spengler1, Christian Kreis1, Stéphane Mesnage2, Hendrik Hähl1, and Simon Foster2 — 1Saarland University, Experimental Physics, D-66041 Saarbrücken — 2University of Sheffield, Krebs Institute, Department of Molecular Biology and Biotechnology, Sheffield S10 2TN, United Kingdom
Protein adsorption is the first step in biofilm formation: Protein films serve as a conditioning layer that enables and affects the attachment of bacteria and other organisms. Hence, the understanding and control of protein layers is an important task that is relevant to life sciences and engineering. Previous studies revealed that the structure and density of adsorbed proteins and the adhesion force of bacteria depend on both the surface properties and the subsurface composition of the adsorbent material [1,2]. These findings raise the question whether or not the activity of adsorbed proteins is also influenced by the properties of the underlying material. In this study, we investigate how the activity – the bactericidal effect – of adsorbed lysozyme and lysostaphin is affected by surface properties. The activity is thereby characterized by measuring the turbidity of a very sensitive protein assay containing purified peptidoglycan.
[1] Hähl et al., Langmuir 28 (2012) 7747-7756
[2] Loskill et al., Langmuir 28 (2012) 7242-7248