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BP: Fachverband Biologische Physik
BP 14: Posters: Protein Structure and dynamics
BP 14.13: Poster
Tuesday, April 1, 2014, 09:30–12:30, P1
Fluorescence Lifetime Spectroscopy of Free and Enzyme-bound NADH — •André Weber1,2, Werner Zuschratter2, and Marcus Hauser1 — 1Abteilung Biophysik, Institut für Experimentelle Physik, Otto-von-Guericke-Universität Magdeburg, Universitätsplatz 2, 39106 Magdeburg, Germany — 2Leibniz-Institut für Neurobiologie, Speziallabor Elektronen- und Laserscanmikroskopie, Brenneckestr. 6, 39118 Magdeburg, Germany
NADH plays a keyrole in energy metabolism of cells and its autofluorescence acts as an indicator for metabolic states of a cell. Certain enzymes, so called dehydrogenases, build up products from substrates by a transport of hydrogen to NAD+.
The fluorescence lifetimes of NADH allow for differentiation between free diffusing and protein-bound NADH and are sensitive to pH, temperature and ionic strength.
We investigate the concentration dependent reaction dynamics of dehydrogenases while binding to NADH and substrates in solution with various pH values via fluorescence lifetime spectroscopy of NADH fluorescence. Through time and space correlated single photon counting we analyse the wavelength dependence of the fluorescence decays.