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BP: Fachverband Biologische Physik

BP 14: Posters: Protein Structure and dynamics

BP 14.7: Poster

Tuesday, April 1, 2014, 09:30–12:30, P1

Ultrafast Side Chain Dynamics of Peptides at the Air-Water Interface — •Michael Donovan, Mischa Bonn, and Tobias Weidner — Max Planck Institute for Polymer Research, Ackermannweg 10, 55128 Mainz, Germany

Proteins possess fluctuating structural forms, and very little is known about their dynamics at the interface between two bulk phases. Few tools are available to study dynamics at interfaces. For this reason we chose to use a time resolved variant of the intrinsically surface specific vibrational spectroscopy Infrared-Visible Sum Frequency Generation. The rotational dynamics of side chains are followed by orthogonal infrared pump pulses and SFG probe pulses. The IR pulse bleaches specific side chain orientations. The recovery of the steady state signal, related to side chain rotational motion, is monitored in real time. Specifically, model amphiphilic LK peptides which consist of alternating leucines and lysines have been probed at the air water interface. Further experiments which utilize isotopically labeled side chains along the peptide backbone will allow us to resolve the rotational dynamics in further detail.