Dresden 2014 – scientific programme

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BP: Fachverband Biologische Physik

BP 20: Cell adhesion, mechanics and migration I

BP 20.8: Talk

Tuesday, April 1, 2014, 15:30–15:45, HÜL 386

modulation of t-lymphocyte adhesion forces by activation with tnf — •qian li¹, constanze lamprecht¹, dieter adam², and christine selhuber-unkel¹ — ¹Biocompatible Nanomaterials, Institute for Materials Science, University of Kiel, Germany — ²Institute of Immunology, University of Kiel, Germany

Integrin-mediated T-lymphocyte adhesion to endothelial cells is a crucial step in the mammalian inflammatory response and the elimination of pathogens. In recent years, the outside-in signalling pathway of integrins in response to the proinflammatory cytokine tumor necrosis factor (TNF) was thoroughly studied. In addition, also an inside-out signalling pathway of integrins in lymphocyte activation by TNF has been reported. How this activation modulates T-lymphocyte adhesion strength and dynamics is still not understood at all. We have chosen a biophysical approach to address this question and applied single-cell force spectroscopy (SCFS) to investigate T-lymphocyte (Jurkat E6-1) cell adhesion to fibronectin, which is naturally present on top of endothelial cell layers. In detail, we approached single Jurkat E6-1 cells to fibronectin-coated surfaces and analyzed cell detachment forces. We found that the addition of TNF significantly increased the maximum adhesion force and detachment energy of the cells, even at sub-second timescales.