Dresden 2014 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 26: Multi-cellular systems and Physics of Cancer
BP 26.2: Vortrag
Mittwoch, 2. April 2014, 12:00–12:15, ZEU 250
Osmotic effects in MDCK model tissues — •Damir Vurnek1, Sara Kaliman1, Matthias Gebhardt2, Florian Rehfeldt3, Katrina Binger2, and Ana-Sunčana Smith1 — 1Institut für Theoretische Physik, Universität Erlangen-Nürnberg — 2Max-Delbrück Center for Molecular Medicine, Berlin — 33rd Institute of Physics-Biophysics, University of Göttingen
The capacity to respond and adapt to changes in the environmental osmotic conditions is vitally important for the functioning of epithelial tissues. We study this response by growing MDCK II model tissues in environments with an increased concentration of mannitol, urea or NaCl. The phase space of tissue viability is established and characterized from isotonic to elevated toxic conditions. Furthermore, we identify the time scales on which the survival, growth and the internal organization of the colony is affected. In young colonies, elevated osmotic conditions suppress the growth. As the age of the colony increases, adaptation takes place, and the colony develops the same morphology as the controls, with the edge at the low and the center at relatively high densities. We show that the appearance of this internal organization is independent of the initial configuration of seeded cells. Apart from the general trends, example of which is the quadratic growth of the area observed in the young colonies, we characterize the osmolyte-and concentration-specific proliferation rates, growth rates and absolute colony sizes, as well as the steady state cell densities. Finally, we analyze the internal structure of cells within the colony and characterize the changes in their nuclei shape and the evident DNA damage.