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BP: Fachverband Biologische Physik
BP 28: Protein structure and dynamics II
BP 28.10: Talk
Wednesday, April 2, 2014, 17:45–18:00, HÜL 386
Adsorptive Capability and Conformational Efficiency between Lysozyme and Nanosilica/-diamond at pH=7-13 — •Victor Wei-Keh Chao — Department of Chemical and Materials Engineering, National Kaohsiung University of Applied Sciences, 80782 Kaohsiung, Taiwan. — Victor Basic Research Laboratory, e.V. Gadderbaumer-Str. 22, D-33602 Bielefeld, Germany.
Adsorption dynamics of lysozyme and nanosilica(NS)/-diamond(ND) with diameter 100 nm and 0.25 µg/µL, lysozyme in 0-1000 nM of 7 mM PPBS at pH=7, 9, 11, and 13 have been investigated by Fluorescence spectroscopy. Chem. instead of phys. adsorption, as well as modification of nanosurface may be necessary for the further investigation of nanocarring of protein or drug through pH-gradients in vivo. Surface of ND was acidified and with approx. 7 % of all covered with COOH groups. Because the acidified surface was only a small part, profile or roughness of the nanosurface is still the decisive factor for the comparison of adsorption strength between NS and ND (adsorption reaction constant NS/ND≈1/4 at four pH values). The highest adsorption capabilities and conformational efficiencies at pH=13 have been obtained. Lysozyme can be prepared, adsorbed and carried with optimal activity and helicity, with 10 and 2 mg/m2 on nanosurface, 150 and 130 mg/g in g of nanoparticle, within the linear coverages at 150-250 nM and four pH values for NS and ND, respectively. They can be prepared in the tightest packed form, with 55 and 20 mg/m2, 580-1100 and 810-1680 mg/g at adsorption thresholds and four pH for NS and ND, respectively. Ref. Chin. J. Chem. Phys. 26, 295(2013).