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BP: Fachverband Biologische Physik
BP 34: Imaging
BP 34.3: Vortrag
Donnerstag, 3. April 2014, 10:00–10:15, ZEU 250
Imaging of biological cells with helium-ion microscopy — •André Beyer1, Natalie Frese1, Henning Vieker1, Matthias Schürmann2, Barbara Kaltschmidt2, Christian Kaltschmidt2, and Armin Gölzhäuser1 — 1Physics of Supramolecular Systems, University of Bielefeld, 33615 Bielefeld, Germany — 2Cell Biology, University of Bielefeld, 33615 Bielefeld, Germany
Helium-ion microscopy (HIM) images are generated by scanning a beam of helium ions while recording the emitted secondary electrons. Advantages of HIM include the high resolution, high surface sensitivity as well as an efficient charge compensation mechanism, which allows imaging of insulating samples without the need for a conductive coating.
In this contribution, a HIM imaging study of biological cells is presented. This study focuses on neuronal differentiated inferior turbinate stem cells as well as mouse neurons which were prepared in different ways for imaging under the required vacuum conditions. Charging of specimens without conductive coating was effectively compensated by an electron flood gun. Therewith, extremely small features at cell surfaces were imaged with an estimated edge resolution of 1.5 nm. Indications of lipid rafts at the surface of all investigated cells will be discussed.