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BP: Fachverband Biologische Physik
BP 38: Active cell and tissue mechanics (focus session) II
BP 38.7: Vortrag
Donnerstag, 3. April 2014, 17:00–17:15, HÜL 386
Individual cell phenotype determines growth modes of cell colonies — •Ben Fabry1, Janina Lange1, Pamela Strissel2, Julian Steinwachs1, and Claus Metzner1 — 1Department of Physics, University of Erlangen-Nuremberg, Erlangen, Germany — 2Women's Hospital, University Clinics, Erlangen, Germany
Many tumor cells proliferate without anchorage to the matrix and often lack the cell-contact inhibition that normally prevents cells from proliferating beyond confluency. It is unclear, however, how individual cells contribute to the collective behavior and growth in a colony. Here we study colonies of different tumor and non-tumor cells lines on planar substrates. Despite the stochastic behavior of individual cells, deterministic features emerge at the colony level that are qualitatively independent of cell type, such as the linear increase of colony radius with time, a global radial streaming motion of cells away from the colony center, and a strong increase of streaming velocity and persistence at the colony border. Quantitatively, however, we find systematic differences between 6 differently adhesive and cohesive cell lines. All measured collective and single cell parameters showed a strong covariance, and no single parameter emerged as a principle component. Rather, all parameters correlated or anticorrelated strongly with the ranking order of these cell lines from a mesenchymal to an epithelial cell phenotype, suggesting that collective behavior is tightly linked with individual cell mechanical behavior.