Dresden 2014 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 5: Protein structure and dynamics I
BP 5.6: Vortrag
Montag, 31. März 2014, 16:15–16:30, ZEU 250
Revealing Rad51 and 53BP1 distribution in HeLa cell nuclei after low and high LET irradiation — •Judith Reindl1, Guido A. Drexler2, Stefanie Girst1, Christoph Greubel1, Christian Siebenwirth1, Sophie Drexler2, Anna A. Friedl2, and Günther Dollinger1 — 1Universität der Bundeswehr, Werner-Heisenberg-Weg 39, 85577 Neubiberg, Germany — 2Ludwig-Maximilians-Universität München, 80336 München, Germany
53BP1 and Rad51 are prominent representatives for two DNA damage repair compartments, the flanking chromatin and the single-stranded DNA (ssDNA) compartment. The correlation between these two repair proteins is revealed by super high resolution STED microscopy with respect to the damage distribution inside a cell nucleus. Ionizing radiation creates double-strand breaks (DSB) of high local density and different complexity with respect to its LET (Linear Energy Transfer). Therefore low LET proton and high LET carbon ion irradiation at the irradiation facility SNAKE are used to generate different damage distributions inside cell nuclei. It is possible to image and analyse the ionizing radiation induced foci (IRIF) in their fine structure and visualize the mutual exclusion of the two damage repair compartments in one cell nucleus by analysing the correlation of Rad51 and 53BP1. Therefore a newly designed analysis program is used, which examines the local correlation of two signalling channels. The presented experimental findings clearly support the two compartment model which could be demonstrated for the first time in one cell for Rad51 and 53BP1.