Dresden 2014 – wissenschaftliches Programm
Bereiche | Tage | Auswahl | Suche | Aktualisierungen | Downloads | Hilfe
BP: Fachverband Biologische Physik
BP 7: Posters: Cell adhesion, mechanics and migration
BP 7.1: Poster
Montag, 31. März 2014, 17:30–19:30, P3
Mechanical Response of MDCK II Cells to Porous Substrates — •Matthias Büchsenschütz-Göbeler1, Jan Rother3, Andreas Janshoff3, Walter Arnold1,2, and Konrad Samwer1 — 1I. Physikalisches Institut, Universität Göttingen — 2Department of Material Science and Materials Technology, Saarland University — 3Institut für Physikalische Chemie, Universität Göttingen
A large number of cellular processes, such as proliferation, differentiation and motility involve adhesion and mechanical adaption of living cells to surfaces. Usually, cells are part of whole cellular networks and connected by an extracellular matrix (ECM). Thereby, elasticity, chemical composition and topography of the ECM affect cell motility and viscoelasticity by changing signal transduction pathways that in turn influence organization of cytoskeleton. The viscoelastic response of living MDCK II cells and adhesion to porous surface elasticity has been investigated by conventional nanoindentation and by a microrheological approach using a modified atomic force microscope. The structure of the cytoskeleton has been monitored by scanning electron microscopy and total internal reflection microscopy. The results show that cells grown on porous substrates are generally softer than cells grown on smooth substrates. With increasing pore size the stiffness of the cells decreases until a certain threshold size is reached. For larger pore sizes the stiffness is increasing again. In addition to this mechanical behavior a correlation with the cytoskeletal structures of the cells is observed.
Financial support by the DFG SFB 937 is thankfully acknowledged.