Dresden 2014 – wissenschaftliches Programm
Bereiche | Tage | Auswahl | Suche | Aktualisierungen | Downloads | Hilfe
BP: Fachverband Biologische Physik
BP 7: Posters: Cell adhesion, mechanics and migration
BP 7.26: Poster
Montag, 31. März 2014, 17:30–19:30, P3
Dynamics of Cellular Force Generation — •Mari Gorelashvili1, Philipp Paulitschke2, Eva Weig3, and Doris Heinrich1, 4 — 1Fraunhofer Institute for Silicate Research ISC, Neunerplatz 2, 97082 Würzburg, Germany — 2Ludwig-Maximilians University Munich, Geschwister-Scholl-Platz 1, 80539 Munich, Germany — 3University of Konstanz, Universitätsstraße 10, 78464 Konstanz, Germany — 4Leiden University, LION, Leiden Institute of Physics, Niels Bohrweg 2, 2333 CA Leiden,The Netherlands
Force generation is one of the basic mechanisms involved in cell-environment interaction. Different biochemical as well as biophysical properties of cellular force exertion have been revealed during the last decade. Nevertheless, simultaneous investigation of these mechanisms during cell migration in 3D environments is less studied. Here, we present a novel method for the investigation of force generation by living cells during the mirgation in quasi 3D environments. The method combines highly precise cellular force measurement and quantitative analysis of cytoskeleton dynamics. Well-defined flexible nanowire arrays serve as force sensors. During cell migration in-between these nanowires cytoskeleton structures and force sensors are imaged simultaneously by spinning disc confocal microscopy. Advanced quantitative analysis algorithms enable determining the force and investigation of underlying cytoskeleton dynamics with high spatial and temporal resolution.