Dresden 2014 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 7: Posters: Cell adhesion, mechanics and migration
BP 7.29: Poster
Montag, 31. März 2014, 17:30–19:30, P3
Fabrication of patterned neuronal networks on multi electrode arrays — •Norman Shepheard1, Stefan Niehörster1, Matthias Schürmann2, Savio Fabretti1, Barbara Kaltschmidt2, Christian Kaltschmidt2, and Andy Thomas1,3 — 1Fakultät für Physik, Universität Bielefeld — 2Fakultät für Biologie, Universität Bielefeld — 3Fachbereich Physik, Johannes Gutenberg Universität Mainz
The geometry of neuronal networks seems to be one of the key features to understand the brain functions. Difficulties to examine these networks are caused by the large amount of connections between neurons. A first step is analyzing networks with reduced complexity, for example in vitro neuronal networks with two or three connected neurons. We fabricated patterned adhesion films for neurons on commercial multi electrode arrays (MEAs). The adhesion film consist of three layers on top of the MEA surface which is made from silicon nitride. These layers are (3-aminopropyl)triethoxysilane (APTES), glutaraldehyde and as top layer poly-L-lysin (PLL) which is covalent bound [1]. Patterning is done by using the UV-lithographic 'lift-off' technique [1]. There are several demands to the geometry of the pattern, like the size of the grid, which has to match the distance between two neurons and which has to fit to the electrodes given by commercial MEAs. We successfully coated the pattern with neurons which is the final test. The aim of the overall project is to compare the behavior of small in vitro neuronal networks and artificial networks build up of memristors.
[1] Yong Hee Kim et al.; J Neurosci Methods. 2011; 202(1):38-44