Dresden 2014 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 7: Posters: Cell adhesion, mechanics and migration
BP 7.42: Poster
Montag, 31. März 2014, 17:30–19:30, P3
Analytical analysis of cell deformation by hydrodynamic forces in microfluidic flow channels — •Alexander Mietke1, Elisabeth Fischer-Friedrich2, Salvatore Girardo1, Philipp Rosendahl1, Stefan Golfier1, Oliver Otto1, and Jochen Guck1 — 1Biotechnology Center, TU Dresden, Tatzberg 47/49, 01307 Dresden, Germany — 2Max Planck Institute for the Physics of Complex Systems, Nöthnitzer Strasse 38, 01187 Dresden, Germany
The mechanical response of cells or tissue to external loadings has gained significant interest during the last decades. A thorough theoretical understanding of the hydrodynamic forces acting on micron-sized objects is an essential prerequisite to probe and determine their mechanical properties. Here we present an analytic formalism to calculate the stresses and ensuing deformations of a spherical viscoelastic object being flowed through a microfluidic channel. Instead of performing full numerical simulations, we describe how a few simplifying assumptions can lead to a comprehensive analytical understanding of the system. This includes the different hydrodynamic stress components and the resulting deformations of the object. Our theory gives direct information about the scaling of shape measures depending on the experimental conditions and the mechanical properties of the object. Computations to determine and visualise flow fields and object deformations can be performed within a second. Finally, we demonstrate that our model is in agreement with experimental data. This analysis forms an important stepping stone for the further development of emerging high-throughput microfluidic cell mechanical phenotyping approaches.