Dresden 2014 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 9: Posters: Biotechnology and bioengineering

BP 9.3: Poster

Montag, 31. März 2014, 17:30–19:30, P3

Bioactive Surfaces by Polymer Pen Lithography — •Ravi Kapoor¹, Falko Brinkmann^1,2, Sylwia Sekula-Neuner¹, Michael Hirtz¹, and Harald Fuchs^1,2 — ¹Institut für Nanotechnologie (INT) & Karlsruhe Nano Micro Facility (KNMF), Karlsruher Institut für Technologie (KIT), 76021 Karlsruhe, Germany — ²Physikalisches Institut & Center for Nanotechnology (CeNTech), Universität Münster, 48149 Münster, Germany

Polymer pen lithography (PPL) is a promising soft lithography technique which has the capability of patterning large areas with precision without denaturing or damaging delicate organic and biologically active compounds. PPL is actually combination of microcontact printing and dip-pen nanolithography, and it takes the advantage of microcontact printing for patterning large areas and dip-pen nanolithography for precisely delivering the ink molecules on the surface. The ink transfer mode is alike microcontact printing or pen spotting approaches, depending on the ink / substrate combination. Multiplexing, i.e. patterning more than one ink compound in close proximity onto the surface is highly demanded in biological applications and can be provided by PPL. Here we present the application of PPL for patterning with different bio-active ink / substrate combinations, e.g. a DNP azide ink on an alkyne-terminated surface with copper catalyzed azide-alkyne cycloaddition (CuAAC). The retained functionality of the DNP allergen head group is confirmed by detection of allergen specific Immunoglobin E (IgE) antibodies. This will offer important improvements for substrates used in the study of mast cell activation in the future.