Dresden 2014 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 9: Posters: Biotechnology and bioengineering

BP 9.5: Poster

Montag, 31. März 2014, 17:30–19:30, P3

Programmable patterning of protein bioactivity on surfaces using visible light — •Cordula Reuther^{1, 2}, Robert Tucker^{2, 4}, Leonid Ionov^{2, 3}, and Stefan Diez^{1, 2} — ¹B CUBE, TU Dresden, Dresden, Germany — ²Max-Planck-Institute of Molecular Cell Biology and Genetics, Dresden, Germany — ³Leibnitz Institute for Polymer Research, Dresden, Germany — ⁴Hansen Medical, Mountain View, California, USA

Patterning functional proteins on engineered surfaces is of interest for the development of nanotechnology, tissue engineering, biosensors and cell biology. Here, we report on the programmable patterning of proteins as well as the local activation of enzymes using patterned wide-field illumination by visible light. Specifically, the light locally heats the carbon-coated surface and switches the conformation of a thermo-responsive poly(N-isopropylacrylamide) (PNIPAM) polymer layer in aqueous solution between the swollen state at T < 30°C (protein-repelling conformation) to the collapsed state at T > 33°C (protein-binding conformation). Thereby, functional protein patterns with different geometries and sizes could be successfully generated in situ. Moreover, we demonstrated the specific patterning of multiple kinds of proteins side-by-side by sequential processing without the need for specific linker molecules or elaborate surface preparation. Additionally, while performing microtubule-based gliding motility assays, the accessibility of kinesin-1 motor proteins could be switched reversibly in a localized manner.