Berlin 2015 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 1: Imaging
BP 1.5: Vortrag
Montag, 16. März 2015, 10:45–11:00, H 1028
Wide-field fast frame-rate FLIM and simultaneous FCS for applications in molecular biology — •Csongor Keuer1, Danilo Bronzi2, Marco Vitali1, Franco Zappa2, Cornelia Junghans1, Thomas Friedrich1, and Franz-Josef Schmitt1 — 1Institute of Chemistry, Bioenergetics, TU Berlin, Straße des 17. Juni 135, D-10623 Berlin, Germany — 2Politecnico di Milano, Piazza Leonardo da Vinci 32, 20133 Milano, Italy
The application of a 64x32 SPAD-based CMOS image sensor for single molecule spectroscopy is presented. Fluorescence amplitudes and lifetimes are measured simultaneously with correlation of fluorescence singal for each pixel. The system is suitable to spatially resolve diffusion constants and concentrations of single molecules in total internal reflection (TIR) excitation of the sample. An image splitter has been added to simultaneously perform both FLIM and FCS of diffusing fluorophores in aqueous solution in two wavelength or polarization channels. The system is suitable for particle size measurement, fast concentration determination and imaging of molecular dynamic processes.